Recent Publications

 

A complete collection of Josh Leonard’s publications can be found at Pubmed or Google Scholar.

---

2024

---

Jung JK, Dreyer KS, Dray KE, Muldoon JJ, George J, Shirman S, Cabezas MD, D’Aquino AE, Verosloff MS, Seki K, Rybnicky GA, Alam KK, Bagheri N, Jewett MC, Leonard JN*, Mangan NM*, Lucks JB (2024). Developing, characterizing and modeling CRISPR-based point-of-use pathogen diagnostics. bioRxiv. *co-corresponding authors. e-pub.

---

Mitrut RE, Stranford DM*, DiBiase BN*, Chan JM, Bailey MD, Luo M, Harper CS, Meade TJ, Wang M, and Leonard JN (2024). HaloTag display enables quantitative single-particle characterisation and functionalisation of engineered extracellular vesicles. Journal of Extracellular Vesicles. *co-second authorship. e-pub.

---

Peruzzi JA, Gunnels TF, Edelstein HI, Lu P, Baker D, Leonard JN*, and Kamat NP* (2024). Enhancing extracellular vesicle cargo loading and functional delivery by engineering protein-lipid interactions. Nature Communications. *co-corresponding authors. e-pub.

---

Dolberg TB, Gunnels TF, Ling T, Sarnese KA, Crispino JD, and Leonard JN (2024). Building synthetic biosensors using red blood cell proteins. ACS Synthetic Biology. e-pub.

---

Edelstein HI, Cosio A, Ezekiel ML, Corcoran WK, Morris AH, and Leonard JN (2024). Conversion of natural cytokine receptors into orthogonal synthetic biosensors. bioRxiv. e-pub.

---

2023

---

Stranford DM, Simons LM, Berman KE, Cheng L, DiBiase BN, Hung ME, Lucks JB, Hultquist JF, and Leonard JN (2023). Genetically encoding multiple functionalities into extracellular vesicles for the targeted delivery of biologics to T cells. Nature Biomedical Engineering. e-pub.

---

Stranford DM and Leonard JN (2023). Engineering cell-derived extracellular vesicles for gene therapy. Nature Biomedical Engineering. e-pub.

---

Dray KE, Dreyer KS, Lucks JB, and Leonard JN (2023). Teaching systematic, reproducible model development using synthetic biology. Chemical Engineering Education. e-pub.

---

Deol S, Donahue PS, Mitrut RE, Hammit-Kess IJ, Ahn J, Zhang B, and Leonard JN (2023). Comparative evaluation of synthetic cytokines for enhancing production and performance of NK92 cell-based therapies. GEN Biotechnology. e-pub.

---

2022

---

Cabrera A, Edelstein HI, Glykofrydis F, Love KS, Palacios S, Tycko J, Zhang M, Lensch S, Shields CE, Livingston M, Weiss R, Zhao H, Haynes KA, Morsut L, Chen YY, Khalil AS, Wong WW, Collins JJ, Rosser SJ, Polizzi K, Elowitz MB, Fussenegger M, Hilton IB, Leonard JN, Bintu L, Galloway KE,  and Deans TL (2022). The sound of silence: Transgene silencing in mammalian cell engineering. Cell Systems. e-pub.

---

Prybutok AN, Yu JS, Leonard JN*, and Bagheri N* (2022). Mapping CAR T-cell design space using agent-based models. Frontiers in Molecular Biosciences. *co-corresponding authors. e-pub.

---

Edelstein HI*, Bora GG*, and Leonard JN (2022). Engineering Mammalian Cells to Communicate Using a Language from Plants. GEN Biotechnology. *co-first authorship. e-pub.

---

Gunnels TF, Stranford DM, Mitrut RE, Kamat N*, and Leonard JN* (2021). Elucidating design principles for engineering cell-derived vesicles to inhibit SARS-CoV-2 infection. Small. *co-corresponding authors. e-pub.

---

Prybutok AN*, Cain JY*, Leonard JN^, and Bagheri N^ (2022). Fighting fire with fire: deploying complexity in computational modeling to effectively characterize complex biological systems. Current Opinion in Biotechnology. *co-first authors. ^co-corresponding authors. e-pub.

---

Manhas J*, Edelstein HI*, Leonard JN, and Morsut L (2022). The evolution of synthetic receptor systems. Nature Chemical Biology. *co-first authors. e-pub.

---

Dray KE, Muldoon JJ, Mangan N, Bagheri N*, and Leonard JN* (2022). GAMES: A dynamic model development workflow for rigorous characterization of synthetic genetic systems. ACS Synthetic Biology. *co-corresponding authors. e-pub.

---

2021

---

Dray KE*, Edelstein HI*, Dreyer KS*, and Leonard JN (2021). Control of mammalian cell-based devices with genetic programming. Current Opinion in Systems Biology. *co-first authors. e-pub.

---

Cable J, Leonard JN, Lu TK, Xie Z, Chang MW, Fernández LÁ, Lora JM, Kaufman HL, Quintana FJ, Geiger R, Lesser CF, Lynch JP, Hava DL, Cornish VW, Lee GK, DiAndreth B, Fero M, Srivastava R, De Coster T, Roybal KT, Rackham OJL, Kiani S, Zhu I, Hernandez-Lopez RA, Guo T, and Chen WCW (2021). Synthetic biology: at the crossroads of genetic engineering and human therapeutics–a Keystone Symposia report. Annals of The New York Academy of Sciences. e-pub.

---

Verosloff MS, Corcoran WK, Dolberg TD, Bushhouse DZ, Leonard JN, and Lucks JB (2020). RNA sequence and structure determinants of Pol III transcriptional termination in human cells. Journal of Molecular Biology. e-pub.

---

Muldoon JJ, Kandula V, Hong M, Donahue PS, Boucher JD, Bagheri N, and Leonard JN (2021). Model-guided design of mammalian genetic programs. Science Advances. e-pub.

---

*Dolberg TB, *Meger AT, Boucher JD, Corcoran WK, Schauer EE, Prybutok AN, Raman S, and Leonard JN (2021). Computation-guided optimization of split protein systems. Nature Chemical Biology. *co-first authors. e-pub.

---

2020

---

Edelstein HI, Donahue PS, Muldoon JJ, Kang AK, Dolberg TB, Battaglia LM, Allchin ER, Hong M, Leonard JN (2020). Elucidation and refinement of synthetic receptor mechanisms. Synthetic Biology. e-pub.

 

---

Nathamgari SSP, Pathak N, Lemaitre V, Mukherjee P, Muldoon JJ, Peng C, McGuire T, Leonard JN, Kessler JA, Espinosa HD (2020). Nano Fountain Probe Electroporation Enables Versatile Single-Cell Intracellular Delivery and Investigation of Post-Pulse Electro-pore Dynamics. Small. e-pub.

 

---

Muldoon JJ, Chuang Y, Bagheri N, and Leonard JN (2020). Macrophages employ quorum licensing to regulate collective activation. Nature Communications. e-pub.

 

 

 

 

---

Donahue PS, *Draut JW, *Muldoon JJ, *Edelstein HI, Bagheri N, and Leonard JN (2020). The COMET toolkit for composing customizable genetic programs in mammalian cells. Nature Communications. *co-second authors.  e-pub.

 

 

---

2018

---

Kang Li, Rodosthenous RS, Kashanchi F, Gingeras T, Gould SJ, Kuo LS, Kurre P, Lee H, Leonard JN, Liu H, Lombo TB, Momma S, Nolan JP, Ochocinska MJ, Pegtel DM, Sadovsky Y,  Sánchez-Madrid F, Valdes KM, Vickers KC, Weaver AM, Witwer KW, Zeng Y, Das S, Raffai RL, and Howcroft TK (2018). Advances, challenges, and opportunities in extracellular RNA biology: insights from the NIH exRNA Strategic Workshop. JCI Insight. e-pub.

 

---

Dolberg TB, Donahue PS, Leonard JN (2018). Synthetic biology: Reframing cell therapy for cancer. Nature Chemical Biology. e-pub

 

---

Hung ME, Lenzini SB, Stranford DM, Leonard JN (2018). Enrichment of Extracellular Vesicle Subpopulations Via Affinity Chromatography. Methods in Molecular Biology. Pubmed

---

Hong BJ, Iscen A, Chipre A, Li MM, Lee OS, Leonard JN, Schatz G, Nguyen SB (2018). Highly Stable, Ultra-Small Polymer-Grafted Nanobins (usPGNs) with Stimuli-Responsive Capability. Journal of Physical Chemistry. Pubmed

 

---

Younger AKD, Su PY, Shepard AJ, Udani SV, Cybulski TR, Tyo KE, Leonard JN (2018). Development of novel metabolite-responsive transcription factors via transposon-mediated protein fusion. Protein Engineering, Design, and Selection. Pubmed

---

2017

---

Nut C, Rodriguez E, Donahue PS, Stranford DM, Leonard JN (2017). An investigation of petty theft of beach decorations at American universities. Journal Criminal Justice. Read more

---

Muldoon JJ, Donahue PS, Dolberg TB, Leonard JN (2017). Building with intent: Technologies and principles for engineering mammalian cell-based therapies to sense and respond. Current Opinion in Biomedical Engineering. Epub ahead of print

---

Xiao X, Wang Y, Leonard JN, Hall CK (2017). Extended Concerted Rotation Technique Enhances the Sampling Efficiency of the Computational Peptide-Design Algorithm. Journal of Chemical Theory and Computation. Pubmed

 

---

Stranford DM, Leonard JN (2017). Delivery of Biomolecules via Extracellular Vesicles: A Budding Therapeutic Strategy. Advances in Genetics. Pubmed

---

Hartfield RM, Schwarz KA, Muldoon JJ, Bagheri N, Leonard JN (2017). Multiplexing engineered receptors for multiparametric evaluation of environmental ligands. ACS Synthetic Biology. Pubmed

---

Stranford DM, Hung ME, Gargus ES, Shah RN, Leonard JN (2017). A systematic evaluation of factors affecting extracellular vesicle uptake by breast cancer cells. Tissue Engineering Part A. Pubmed (Invited contribution to special issue)

 

---

Schwarz KA, Daringer NM, Dolberg TB, Leonard JN (2017). Rewiring human cellular input–output using modular extracellular sensors. Nature Chemical Biology. Pubmed

 

---

2016

---

Younger AKD, Dalvie NC, Rottinghaus AG, Leonard JN (2016). Engineering modular biosensors to confer metabolite-responsive regulation of transcription. ACS Synthetic Biology. Pubmed

 

---

Du Lac M, Scarpelli AH, Younger AKD, Bates DG, Leonard JN (2016). Predicting the dynamics and heterogeneity of genomic DNA content within bacterial populations across variable growth regimes. ACS Synthetic Biology. Pubmed

 

---

Chuang YC, Hung ME, Cangelose BK, Leonard JN (2016). Regulation of the IL-10-driven macrophage phenotype under incoherent stimuli. Innate Immunity. Pubmed

 

---

Xaio X, Hung ME, Leonard JN, Hall CK (2016). Adding energy minimization strategy to peptide-design algorithm enables better search for RNA-binding peptides: Redesigned λ N peptide binds boxB RNA. Journal of Computational Chemistry. Pubmed

 

 

---

Schwarz KA, Leonard JN. (2016) Engineering cell-based therapies to interface robustly with host physiology. Advanced Drug Delivery Reviews. Pubmed

 

---

Hung ME, Leonard JN (2016). A platform for actively loading cargo RNA to elucidate limiting steps in EV-mediated delivery. Journal Extracellular Vesicles. Pubmed

 

---

Liu JM, Zhang J, Zhang X, Hlavaty KA, Ricci CF, Leonard JN, Shea LD, Gower RM. (2016) Transforming growth factor-beta 1 delivery from microporous scaffolds decreases inflammation post-implant and enhances function of transplanted islets. Biomaterials. Pubmed